Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• interaction in malignant cell lines. Here, we report on the influence of polymeric nanoparticles on human hematopoietic stem cells (hHSCs) and mesenchymal stem cells (hMSCs). In this study we systematically investigated the influence of polymeric nanoparticles on the cell functionality and differentiation

• analyzed with lineage specific cluster of differentiation markers. On the other hand qPCR analysis showed significant changes in the expression of some (but not all) investigated lineage markers for both primary cell types. Keywords: cytokine secretion; differentiation; hematopoietic stem cells

• in [19][20]. Particle characteristics are given in Table 1. Isolation of human hematopoietic stem cells (hHSCs) hHSCs were obtained via apheresis from peripheral blood (peripheral blood stem cells, PBSC) for validation purposes of another study after obtaining informed consent. The apheresis product